Invited review: "Probiotic" approaches to improving dairy production: Reassessing "magic foo-foo dust".

The gastrointestinal microbial consortium in dairy cattle is critical to determining the energetic status of the dairy cow from birth through her final lactation. The ruminant's microbial community can degrade a wide variety of feedstuffs, which can affect growth, as well as production rate and efficiency on the farm, but can also affect food safety, animal health, and environmental impacts of dairy production. Gut microbial diversity and density are powerful tools that can be harnessed to benefit both producers and consumers. The incentives in the United States to develop Alternatives to Antibiotics for use in food-animal production have been largely driven by the Veterinary Feed Directive and have led to an increased use of probiotic approaches to alter the gastrointestinal microbial community composition, resulting in improved heifer growth, milk production and efficiency, and animal health. However, the efficacy of direct-fed microbials or probiotics in dairy cattle has been highly variable due to specific microbial ecological factors within the host gut and its native microflora. Interactions (both synergistic and antagonistic) between the microbial ecosystem and the host animal physiology (including epithelial cells, immune system, hormones, enzyme activities, and epigenetics) are critical to understanding why some probiotics work but others do not. Increasing availability of next-generation sequencing approaches provides novel insights into how probiotic approaches change the microbial community composition in the gut that can potentially affect animal health (e.g., diarrhea or scours, gut integrity, foodborne pathogens), as well as animal performance (e.g., growth, reproduction, productivity) and fermentation parameters (e.g., pH, short-chain fatty acids, methane production, and microbial profiles) of cattle. However, it remains clear that all direct-fed microbials are not created equal and their efficacy remains highly variable and dependent on stage of production and farm environment. Collectively, data have demonstrated that probiotic effects are not limited to the simple mechanisms that have been traditionally hypothesized, but instead are part of a complex cascade of microbial ecological and host animal physiological effects that ultimately impact dairy production and profitability.

Nutrient digestibility and nitrogen balance in different pig breeds fed raw, sprouted, or roasted (Vigna unguiculata) diets.

The study examined effects of feeding iso-nutrient (150 g CP, 17.3 MJ ME kg-1) raw (RCP), sprouted (SPC), or roasted (RSCP) cowpea diets to Windsnyer (W), Large White (LW) × Landrace (LR), and the 3-way crossbred (W × LW × LR) growing pigs. Diet dry matter (DM) digestibility was estimated using standard, 3-step (gastric, small intestines, colon) in vitro digestion. Dietary in vivo nutrient digestibility and nitrogen balance were evaluated using 3 weaned pigs of each genotype. Pigs were housed in individual metabolic cages. The diets were assigned to pigs in a 3 × 3 change-over factorial experiment within three balanced, 3 × 3 Latin squares. Feeding periods consisted of 7 days adaption + 5 days' measurement of feed intake, and the total faecal and urine excretions. The SPC diet increased step 3 in vitro DM digestibility compared to RSCP (P < 0.05). Metabolic size-scaled feed consumption was higher on the RCP compared to the RSCP diet (P < 0.05). Cowpea processing reduced apparent DM and ash digestibility (P < 0.05). LW × LR pigs exhibited lower digestibility of ash and acid detergent fiber (ADF) compared to 3-way crossbred pigs (P < 0.05). Significant genotype-diet interactions were observed for nitrogen intake (P < 0.0001), digestible nitrogen (P = 0.043), urinary nitrogen output (P < 0.0001), faecal nitrogen output (P < 0.0001), total nitrogen excretion (P < 0.0001), and nitrogen retention (P < 0.001). The biological value of feed protein was higher for W pigs than 3-way crossbred pigs (P < 0.05). Genotype-diet interactions suggested unique digestive and, or metabolic adaptive traits in the utilization of the differently processed cowpeas, which need further investigation.

Research Note: Responses of broiler chickens to in ovo feeding with clove and cinnamon extract under hot-humid environments.

An experiment was carried out to evaluate the responses of broiler chickens to in ovo injection of aqueous extracts of clove and cinnamon under a hot-humid environment. The study involved the use of seven hundred hatching eggs from broilers (Ross 308) which were incubated with the use of standard protocol (37.8°C). The incubating eggs (100 each) were randomly selected and assigned to 7 treatments on day 17.5 of incubation, viz.: un-injected eggs (UE), eggs injected with 0.5 mL distilled water (DW), 2 mg clove (CL2), 4 mg clove (CL4), 2 mg cinnamon (CN2), 4 mg cinnamon (CN4), and 3 mg ascorbic acid (AA).Data on physiological parameters, hatchability, chick quality, and anatomical characteristics of the chicks were collected and analyzed using one-way analysis of variance. The results obtained revealed that the hatchability of eggs of AA and CN2 was higher compared to DW and UE. However, the hatchability of DW and UE was higher than those of CN4. The total chick quality scores of the control were similar to the other groups. Chick weights at hatch were similar in CL2, CN2, and AA but heavier than CN4, CL4, UE, and DW. The chick-to-egg ratio in AA was comparable to CL2 and CN2 but higher than UE, DW, CN4, and CL4. Total scores for chick quality of AA birds were similar to those of UE, CL2, and CL4 birds but higher than DW, CN2, and CN4 birds.

Adaptation of the White Fulani cattle to the tropical environment.

This review summarizes the available information on the adaptation and the performance of White Fulani cattle in tropical environments. White Fulani cattle is an indigenous breed of cattle widely distributed in the humid tropical regions of Africa and plays significant roles in meat and milk production, as well as draught purposes. Poor management and the harsh environmental conditions in the tropics encumber the optimal productive and reproductive performance of the breed, having about 4.75 years age at first calving, 57% calving rate, 18 months calving interval and 3 to 4 calves are born in a reproductive lifetime. The cattle breed adapts to these climatic patterns via physiological, morphological, behavioral, genetic, and metabolic responses. Different physiological mechanisms and morphological features such as their white coat color, hair coat thickness, and lengthy rump appear to play an important adaptive role. The distinct genetic traits (including the possession of seven genetic variants of HSP 90 gene) of the breed afford them some levels of thermotolerance and high resistivity to some endemic diseases (such as trypanosomiasis, liver fluke, brucellosis and foot and mouth disease) in the tropics constitute inherent characteristics that should be explored in detail using molecular assisted approaches. The productive performance of the breed under different systems is discussed. A better understanding of the adaptive characteristics of White Fulani cattle could provide crucial information on the best management approach/techniques that should be adopted to improve the performance, productivity, and sustainability of this cattle breed. Therefore, this review aims to compile the various production, reproduction and adaptation traits of the breed and presents vital information underlying their thriving and survivability in tropical environments.

The effect feeding forage legumes as nitrogen supplement on growth performance of sheep.

The effect of feeding forage legumes, Cowpea, Silverleaf desmodium and Oxley fine stem stylo, as protein supplements to natural pasture (veld) hay on intake, growth rate and nitrogen metabolism in growing lambs was evaluated. Thirty growing lambs were stratified according to body weight and randomly assigned, within a stratum, to five diets in a completely randomised design. The diets were veld hay alone (V), veld hay supplemented with either 10 g/kg of urea (VU), veld hay supplemented with 250 g/kg Cowpea (VC), 250 g/kg Silverleaf desmodium (VS) or 250 g/kg Oxley fine stem stylo (VF) forage legume hays. The V and the VU groups were used as control diets. Animals supplemented with either urea or the forage legume had higher (P < 0.01) total dry matter intake compared with the animals on V. The animals supplemented with the forage legumes had higher (P < 0.01) nitrogen intake and faecal nitrogen output than the non-supplemented group. All animals, across the treatments, lost body weight; lambs on V had higher (P < 0.01) body weight losses than those in the other treatments. The forage legume supplemented groups lost less (P < 0.01) body weight than those on the V and VU diets. Although supplementation with forage legumes enhanced feed intake and reduced weight losses it did not maintain body weights of lambs fed a basal diet of poor quality roughages.

Condensed tannin and saponin content of Vigna unguiculata (L.) Walp, Desmodium uncinatum, Stylosanthes guianensis and Stylosanthes scabra grown in Zimbabwe.

Samples of the tropical forage legumes Vigna unguiculata (L.) Walp (cowpea), Desmodium uncinatum (silverleaf desmodium), Stylosanthes guianensis (oxley fine stem stylo) and Stylosanthes scabra (fitzroy) and of natural pasture (veld) hay were analysed and ranked according to their proanthocyanidin (PA) and saponin content. Silverleaf desmodium and fitzroy leaf and stem samples of different ages were also separately analysed for the PA contents. All the samples analysed contained some PA but no saponins. High levels of PA were detected in silverleaf desmodium and very low levels in veld hay and cowpea. In all samples, more of the tannins were bound to protein or neutral detergent fibre (NDF) than were extractable, most being bound to proteins. The proportion of the unextractable PA was greater in younger than in mature materials.